DU Jun-kai1,SHI Jing-sen1,XU Yi-zhuang2,ZHANG Yuan-fu2,SUN Xue-jun1,WANG Jian-sheng1,WENG Shi-fu2, WU Jin-guang2
1. The First Affiliated Hospital, College of Medicine, Xi’an Jiaotong University, Xi’an 710061, China 2. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
Abstract：The aim of this research is to establish the cell basis for the carcinoma tissue diagnosis by exploring a method to obtain the FTIR (Fourier transform infrared) spectra of the cultured carcinoma cells with FTIR spectroscopy and investigating the special spectral features of the carcinoma cells compared with the carcinoma tissues. In the present paper, the gastric carcinoma tissues confirmed by histology were measured using a Nicolet Magna750-Ⅱ FTIR spectrometer and the corresponding FTIR spectra were obtained. The cultured gastric carcinoma cells (SGC7901) were centrifuged to provide a small pellet of cells for FTIR analysis. The cell pellet was then placed on a specially designed salt plate made of BaF2. Then the infrared spectra were recorded by the same equipment. Based on the previously established criteria, a comparative study was subsequently carried out between the spectra of the cultured carcinoma cells (SGC7901) and that of the corresponding gastric tissues. Several infrared spectral features of the carcinoma cells were obtained：the different bands between cells and tissues locate in the range of 3 000-3 600 cm-1 and 1 640 cm-1 which are the range of the hydroxy stretching and blending bands of H2O. There are more H2O out of carcinoma cells in carcinoma tissues，so the strong bands of H2O cover the distinctive bands of carcinoma cells in carcinoma tissues. Although the carcinoma tissue is more complicated, which might originate from the intrinsic complexity of the tissue, the results suggest that the spectral features of the carcinoma cells can be well reflected by that of the carcinoma tissue. This study shows that the diagnosis of carcinoma tissue by FTIR method exhibits sufficient cell basis.
[1] XU Yi-zhuang, ZHAO Ying, XU Zhi, et al(徐怡庄, 赵 莹, 徐 智, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2005,25(11): 1775. [2] YANG Li-min, XU Zhi, ZHANG Yuan-fu, et al(杨丽敏, 徐 智, 张元福, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2003,23(5): 883. [3] SHI Jing-sen, WANG Jian-sheng, XU Yi-zhuang, et al(石景森, 王健生, 徐怡庄，等). Digestive Surgery(消化外科),2003,2(2): 96. [4] Peter R Griffiths, YANG Hu-sheng, LI Qing-bo, et al(Peter R Griffiths, 杨虎生, 李庆波, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2004,24(9): 1025. [5] LI Qing-bo, SUN Xue-jun, XU Yi-zhuang, et al. World Journal of Gastroenterology,2005, 11(25): 3842. [6] WU Jin-guang, XU Yi-zhuang, SUN Chuan-wen, et al. Biopolymers,2001, 62(4): 185. [7] LING Xiao-feng, XU Yi-zhuang, WANG Li-xin, et al(凌晓锋, 徐怡庄, 王立新，等). Chemical Journal of Chinese Universities(高等学校化学学报)，2007,28(3): 445. [8] Benedetti E, Palatresi M R, Vergamini P, et al. Appl. Spectrosc., 1986, 40: 39. [9] PENG Qing, Soloway R D, WU Jin-guang, et al. Gastroenterology, 1996, 110: A576. [10] Zhao D W, Zhou X S, Soloway R D, et al. Gastroenterology, 1996, 110: A620. [11] WANG H P, WANG H C, HUANG Y J. The Science of The Total Environment, 1997,204: 283. [12] Michael Fung Kee Fung, et al. Gynecologic Oncology, 1997, 66: 10. [13] Christian P Schultza, Kan-Zhi Liua, Elizabeth A Salamonb, et al. Journal of Molecular Structure, 1999, 480: 369. [14] LI Wei-xiu, ZHENG Quan-qing, WANG Ping, et al(李炜修, 郑全庆, 王 平, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2006,26(10): 1833. [15] Benedetti E, Teodori L, Trinca M L, et al. Applied Spectroscopy, 1990, 44: 1276. [16] PENG Qing, XU Yi-zhuang, LI Wei-hong, et al(彭 卿, 徐怡庄, 李维红, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，1998, 18(5): 528. [17] HUO Hong, WANG Xing-fu, CHE Xun, et al(霍 红, 王幸福, 车 迅, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2001,21(5): 614.
